Arabidopsis plants use a gatekeeper protein to shuffle Vitamin B2 into their reproductive organs.
Vitamin B2 is essential for all life, yet the mechanism for how plants move it across their cell membranes remained a total mystery until now. The AtPUP5 protein is the first genetically defined transporter found to handle the distribution of this vital nutrient. Without this transporter, the plant cannot properly feed its developing seeds or manage its internal energy cycles. This discovery fills a massive gap in the basic blueprint of plant physiology and nutrient transport. Plant breeders can now look for similar transporters in food crops to potentially increase their vitamin content.
Purine permease 5 contributes to riboflavin distribution in Arabidopsis reproductive organs
bioRxiv · 10.64898/2026.02.25.708111
Riboflavin (vitamin B2; RF) and its derivatives flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are indispensable cofactors for redox reactions in plants. While higher plants possess a conserved pathway for de novo riboflavin biosynthesis, how flavins are transported and spatially distributed between tissues remains unresolved. In particular, no genetically defined plasma membrane-localized flavin transporter has been identified in plants. Here, we identify Arabidopsis PURINE P